A chimeric construct of foot and mouth disease virus capsid protein VP1 was expressed in
Chlamydomonas reinhardtii via Agrobacterium-mediated genetic transformation. Integration of the foreign gene in
nuclear genome of the algae was confirmed by PCR and expression of the recombinant protein was confirmed at
translational level. Results showed that the foreign gene was integrated in nucleus and the recombinant protein was
produced in the microalgal cells. Transformation process together with detection of recombinant protein are
discussed.
کلید واژگان :
genetic transformation, Foot and Mouth Disease, chlamydomonas, recombinant vaccine, VP1
