Objective: In previous studies, we evaluated antibacterial activity of CM11 peptide against multiple drug resistant clinical isolates of six bacteria species, alone and in combination with conven-tional antibiotics. Although antimicrobial peptides like CM11 peptide are one of the best new alterna-tive antibiotics, the side effects such as toxicity are major problem. Previously the in vitro cytotoxic ef-fects of the peptide, viability of some cell lines have been investigated. : MethodsFor in vivo studies, the present study is conducted to obtain more clarification about the tox-icity and histopathological effects of peptide in mice. For this purpose three groups of mice were treated with different concentrations (0.2, 2.5, and 7.5 mg/kg) of peptide and then histological study for the liv-er, kidney and jejunum and aspartate and alanine aminotransferase activities and total serum bilirubin and albuminin blood plasma levels were measured. : Results Our study showed that the activities of aminotransferase, also total serum bilirubin and albu-min levels were not significantly increased while different histological changes were observed in or-gans’ tissue. These changes were dependent on peptide concentration so that an increase in focal in-flammation was observed with increasing peptide concentration; however these histological changes are not considerable in lower concentration. Conclusion: Our findings showed that although in comparison with the control group, markers level is normal but damages were visible in the tissues, as increase in the level of tissue damages has not led to increased levels of markers. It seems that the lack of correlation between tissue damage and level of liv-er markers probably is related to the level of damage.
کلید واژگان :Antimicrobial peptide, CM11 peptide, Cytotoxic effect, Histopathology, Enzyme activity
ارزش ریالی : 600000 ریال
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