The Possibility of Diabetic Wound Healing Using Electro spun PLA Nano Fibers

Diabetes is a metabolic disorder demonstrated by hyperglycemia and is typically resulted from defects in insulin secretion and impaired pancreatic function. In general, wound infection is a common complication of diabetes and finding effective remedies (medications) and methods of diabetic wound healing has recently been of interests of researchers. In the present research study, PLA NANO fiber scaffolds are prepared by electro spinning in two different concentrations of 2% and 4%. Indeed, electron microscopic scanning of this NANO fiber represents the achievement of NANO fibers with average diameter of 190 nm ± 44/4 nm and 460 nm ± 75 nm that encompass a uniform morphology. Given the fact that the PLA NANO fiber scaffolding with concentration of 2% has less average diameter, smaller pores and higher biodegradability, hence, it has been selected to study wound healing. In this study, 18 male Wistar rats weighing 250-280g are divided into three groups. First, two groups of rats become diabetic with streptozotocin (55mg / kg i.p.) and another group is monitored as a control type (healthy rats). After observing the symptoms of diabetes and hyperglycemia (250-300 mg /dl in diabetic group), a wound is made on the back of neck of all four groups with diameter of 1 cm through surgical scissors. Then, wound is treated in the experimental group on a daily basis for 14 days by PLA NANO fiber scaffolding. Meanwhile, macroscopic and microscopic studies are adopted on 0, 4, 7, 14 and 7, 14 days respectively. The results revealed wound size reduction, epidermal gap decrement and reduction in wound treatment area in the treatment group compared to it in healthy groups. This, in turn, implies the positive impact of the NANO fibers in the process of wound healing. Keywords: Diabetic; Wound healing; PLA; Nano fibers; Electro spinning

Diabetic; Wound healing; PLA; Nano fibers; Electro spinning