Background and aims: Clostridium difficile (C. difficile) is a gram-positive, toxin-producing bacillus that can infect both human and animals. The aim of present study was to investigate the frequency of virulence genes and evaluation of antibiotic resistance among isolated C. difficile from feces of calves in Chaharmahal va Bakhtiari province. Methods: In this cross-sectional study, 150 fresh feces of calves were collected and C. difficile was isolated from feces of calves using bacterial culture methods. Bacteria genomic DNA was extracted by a DNA extraction kit, and tcdA and tcdB genes were identified by Multiplex PCR method. In the final stage, antimicrobial resistance was carried out by standard Bauer-Kirby disk diffusion method. Results: 90 samples (60%) were included C. difficile, 8 samples (8.8%) tcdA and 16 samples (17.7%) tcdB. The highest rate of antibiotic resistance was for Clindamycin (100%) and Erythromycin (90%), respectivly. In addition, the highest rate of antibiotic sensitivity was for Ciprofloxacin (50%) and Vancomycin (20%). Conclusion: According to the results of present study, the prevalence and antibiotic-resistant of C. difficile was high. It should be adapted to appropriate methods of prevention and treatment, as well as restricting the use of antimicrobial drugs in the development of human and animal bacteria, especially, pathogenic strains. Investigation of antibiotic resistance and frequency of Clostridium difficile tcdA and tcdB genes in feces of calves in Chaharmahal Va Bakhtiari province (PDF Download Available). Available from: http://www.researchgate.net/publication/277666418_Investigation_of_antibiotic_resistance_and_frequency_of_Clostridium_difficile_tcdA_and_tcdB_genes_in_feces_of_calves_in_Chaharmahal_Va_Bakhtiari_province [accessed Oct 22, 2015].
کلید واژگان :Clostridium difficile, tcdA and tcdB genes, Antibiotic resistance, Calves.
ارزش ریالی : 600000 ریال
با پرداخت الکترونیک