A fast, simple and sensitive uric acid sensor was prepared by nano resin. The modified electrode offers a considerable improvement in voltammetric sensitivity toward uric acid, compared to the bare electrode. Lewatit FO36 nano resin is a macroporous, mono dispersed, weakly basic, polystyrene-based resinwith graphite powderas new modified carbon paste electrode (CPE/LFONR) was used for the electroanalytical determination of uric acid (UA) in real samples. The variation of peak current with pH and scan rate, were optimized. The electron transfer coefficient and diffusion coefficient of UA at the modified electrode were calculated. The electrochemical behavior of UA at CPE/LFONR was studied by cyclic voltammetry (CV), linear sweep voltammetry (LSV), Chronoamperometry (CA) and Chronocoulometry (CC). Under the optimum conditions (pH and scan rate), a linear dependence of the uric acid oxidation signals was observed to concentration in the ranges of 0.3-3.1 μmol L-1 and 3.1- 32.0 μmol L-1 with a detection limit (3s) of 0.176 μmol L-1 at pH 6.0. Using chronoamperometry and Chronocoulometry, diffusion coefficient was calculated 3.70 × 10-6 and 5.91 × 10-6 cm2/s respectively. The results indicate that the proposed method is highly sensitive for uric acid detection in real samples such as human urine and human blood serum.
کلید واژگان :Modified carbon paste electrode • Linear sweep voltammetry • Lewatit FO36 nano resin • Uric acid
ارزش ریالی : 350000 ریال
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