Mycobacterium avium subspecies of paratuberculosis, called Mycobacterium paratuberculosis causes Johnes disease. This bacterium is a common infection factor between human and animals which is widespread throughout the world and requires a reliable method for prevention and control. The purpose of this study is to recognize genotypes of Mycobacterium avium subspecies paratuberculosis isolated from dairy flocks in Tehran province. In these research 100 cattle stools that suspicious to be infected by johns disease extracted. Then, Nested PCR reaction was arranged and carried out by using specific primers of IS900 gene and PCR products gained were electrophoresis on Agarose gel. Then the approved samples were PCR processed by using specific gene primers of GyrA and GyrB. From among positive cases isolated, 20 samples randomly selected were sequence. Then the resulted sequences were recorded in a gene bank and a phylogenic tree diagram was drawn for it using Bioedit and DNA Star software. Based on results of this study and by presenting two genes of GyrA and GyrB, we observed that resulted from our research are similar to that recorded in gene bank. According to the results obtained from observed that separation of MAP-GyrA-spain and MAP-GyrA- Iran was very similar and MAP-uk has had the biggest differences with separations of in GyrA gene. GyrB gene is most similar to the strains of MAP-GyrB-USA and the biggest difference is related to MAP- k10-USA.
کلید واژگان :Mycobacterium avium subspecies paratuberculosis, Johnes disease, Genotype
ارزش ریالی : 300000 ریال
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