Background & Objective: Ischemia and Reperfusion (I/R) lead to the production of excessive reactive
oxygen species (ROS). In the present study, the protective effects of the cell culture conditioned
medium (CM) on sperm quality disorder induced by torsion-detorsion (T-D) in adult rat’s testis was
evaluated.
Materials & Methods: In this study, 42 adult rats were divided into seven groups. 1- Normal control
group treated without surgery. 2- Laparotomy group, surgery operated without T-D. 3- T-D group,
surgery operated along with T-D. 4- T-D+DMEM group, after T-D Dulbecco's Modified Eagle's
Medium (DMEM) has been injected. 5-7 groups included T-D+F, T-D+M, and T-D+FM, along with TD
injected conditioned medium of fibroblast, macrophage, and fibroblast-macrophage co-culture
respectively. All injections were made at a rate of 10μL once after T-D in the rete testis. After 35 days,
sperm quality parameters were evaluated and obtained data were compared between groups by oneway
ANOVA statistic method and Tukey test post hoc at P<0.05 level.
Results: The experimental groups that received CM of the fibroblast and macrophage compared to
groups T-D and T-D+DMEM showed ameliorative effects in comparison with T-D and T-D+DMEM
groups. No ameliorative effects were observed in the T-D+FM group.
Conclusion: The results of this study showed the high ability of supernatant of the separate fibroblast
and macrophage culture in improving sperm parameters after testicular T-D.
